The Institute in Würzburg has a tract record of internationally acclaimed research on the biotransformation of nephrotoxicants and studies related to interactions of biological reactive intermediates with cellular macromolecules and function. Part of this is based on the application of in vitro models (freshly isolated renal cell preparations, renal cell culture, subcellular fractions and isolated perfused organs) to study mechanisms of toxicity.
The laboratory is funded by the Deutsche Forschungsgemeinschaft, the Bundesministerium für Forschung und Technologie, Umweltbundesamt, US EPA, US HEI, industry and a number of foundations. The equipment is at the cutting edge of technology and includes gas chromatography-mass spectrometry with different ionisation techniques, electrospray mass spectrometry (MS/MS), high performance liquid chromatography with fluorescence, diode array and UV-detectors, and a full array of in vitro techniques. Chemical and radiochemical synthesis and a 600 MHz (1H)nuclear magnetic resonance spectrometer is available on the campus.
The laboratory is focusing on the application of mechanistic toxicology to risk assessment of synthetic chemicals. Most aspects of the work performed use analytical procedures (gas chromatography / mass spectrometry and HPLC) to quantify xenobiotic metabolites in biological systems ranging from enzymatic incubations to occupationally exposed humans. Moreover, the laboratory uses biochemically methods to characterise enzymes involved in the biotransformation of xenobiotics.
- Biomonitoring of musk xylene in humans (Deutsche Forschungsgemeinschaft)
- Mechanisms of renal tumorigenicity of polyhalogenated olefins (Deutsche Forschungsgemeinschaft)
- Comparative biotransformation of perchloroethene (US Environmental Protection Agency)
- Liver enzyme induction at the workplace (Biomed-2 concerted action of the European comission)
- Biotransformation of fuel oxygenates (Health Effects Institute, USA)
- Development of new procedures in risk assessment of chemicals (German ministry of Science and Technology)
- Bioactivation and mutagenicity of ocharatoxin A (European Coffeeindustry)
- DNA-binding of ochratoxin A investigated by accelerator mass spectrometry (European Coffeeindustry)
- Hemoglobin adducts of dinitropyrenes as a marker for Diesel emission exposure in humans (German Automobile Industry)
- Mechanisms of ochratoxin A induced carcinogenicity as a basis for an improved risk assessment (5th RTD Framework Programme of the European Union, Project No.: QLK1-2001-01614)
- Risk Assessment of Brominated Flame Retardants as Suspected Endocrine Disrupters for Human and Wildlife Health (5th RTD Framework Programme of the European Union, Project No.: QLK4-CT-2002-00596)
- Covalent Binding of Reactive Metabolites in Conjunction with Other Risk Factors such as Oxidative Stress as Potential Toxicity Mechanisms (Hoffmann-La Roche, Basel)
- In vitro toxicity testing: New approaches for chronic toxicity prediction (RCC Ltd. Itingen)
- Innovative Medicines for Europe - InnoMed (with A. Mally) (European Union, 6th Framework Programme)
- Molecular mechanisms of toxicity and carcinogenicity of the mycotoxin ochratoxin A (with A. Mally) (Deutsche Forschungsgemeinschaft - DFG)
- Biotransformation and toxicokinetics of acrylamide in humans (Deutsche Forschungsgemeinschaft - DFG)
- Role of genetic and non-genetic mechanisms in furan risk (6th Framework Programme of the European Union, Project No.: SSPE-CT-2006-44393)
- Furan in food: a dietary risk factor for human cholangiocarcinoma? Characterization of
functional changes and protein adducts in the hepatobiliary tract of rats exposed to furan (with A. Mally) (Deutsche Forschungsgemeinschaft - DFG)
- Profiling the toxicity of new drugs: a non animal-based approach integrating toxico-dynamics and biokinetics (7th Framework of the European Union, Project No. FP7-HEALTH-2007-A-202222)